Immunolocalization of Sarcoplasmic Reticulum Proteins in Mammalian Skeletal Muscle Fibers
نویسندگان
چکیده
منابع مشابه
Calsequestrin targeting to sarcoplasmic reticulum of skeletal muscle fibers.
Calsequestrin (CS) is the low-affinity, high-capacity calcium binding protein segregated to the lumen of terminal cisternae (TC) of the sarcoplasmic reticulum (SR). The physiological role of CS in controlling calcium release from the SR depends on both its intrinsic properties and its localization. The mechanisms of CS targeting were investigated in skeletal muscle fibers and C2C12 myotubes, a ...
متن کاملEffect of clenbuterol on sarcoplasmic reticulum function in single skinned mammalian skeletal muscle fibers.
We examined the effect of the β2-agonist clenbuterol (50 μM) on depolarization-induced force responses and sarcoplasmic reticulum (SR) function in muscle fibers of the rat ( Rattus norvegicus; killed by halothane overdose) that had been mechanically skinned, rendering the β2-agonist pathway inoperable. Clenbuterol decreased the peak of depolarization-induced force responses in the extensor digi...
متن کاملRole of sarcoplasmic reticulum junctional proteins in skeletal muscle strength
Background Skeletal muscle constitutes approximately 40% of body mass, and age-induced decrease of muscle strength impinge on daily activities and on normal social life in the elderly. Loss of muscle strength has been recognised as a debilitating and life threatening condition also in cachexia in cancer patients and in clinical conditions associated with prolonged bed rest. Skeletal muscle dihy...
متن کاملMicroarray profiling of skeletal muscle sarcoplasmic reticulum proteins.
Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), tr...
متن کاملThe sarcoplasmic reticulum of smooth muscle fibers.
The ability of the sarcoplasmic (endoplasmic) reticulum (SR, ER) of smooth muscle cells to accumulate Ca was demonstrated by measuring the uptake of 45Ca in fibers which were chemically skinned with saponin, and by electron cytochemistry of the accumulated Ca. The Ca uptake was dependent on ATP and it was stimulated by oxalate, as it is the case in SR of striated muscle. Electron microscopy of ...
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ژورنال
عنوان ژورنال: American Zoologist
سال: 1987
ISSN: 0003-1569
DOI: 10.1093/icb/27.4.1021